The influence of rootstock and extraction setting on the Limonin and Flavonoids levels in orange juice during ripeness

The influence of rootstock, maturity and extraction settings on limonin, hesperidin narirutin levels orange juice was evaluated. A liquid chromatographic method to determine limonin developed validated. linear range 0.410 61.5 µg.mL-1, with a correlation coefficient higher than 0.999. limit detection 0.144 µg.mL-1and quantification 0.363 µg; precision showed RSD≤5.0% accuracy from 92.6 100.4%. Limonin identified in Pêra-Rio juices Cleopatra mandarin Rangpur lime rootstocks extracted the NFC FCOJ during 2013 harvest. ranged 0.86 3.94 µg.mL-1 flavonoids 12.00 26.02 for 122.12 175.01 hesperidin. Principal component analysis able differentiate according ripeness, as well settings. flavonoid reduced maturation. were more expressive at beginning harvest, especially obtained setting. Hesperidin about ten times all juices.